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Silver oxide nanoparticles embedded silk fibroin spuns: Microwave mediated preparation, characterization and their synergistic wound healing and anti-bacterial activity

Babu, PJ and Doble, M and Raichur, AM (2018) Silver oxide nanoparticles embedded silk fibroin spuns: Microwave mediated preparation, characterization and their synergistic wound healing and anti-bacterial activity. In: Journal of Colloid and Interface Science, 513 . pp. 62-71.

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Official URL: https://doi.org/10.1016/j.jcis.2017.11.001


The synergistic wound healing and antibacterial activity of silver oxide nanoparticles embedded silk fibroin (Ag2O-SF) spuns is reported here. UV–Vis spectro photometric analysis of these spuns showed the surface plasmon resonance (SPR) confirming the formation of the silver oxide nanoparticles (Ag2O NPs) on the surface of the silk fibroin (SF). Scanning electron microscope (SEM) and Differential scanning calorimetry (DSC) also confirmed the presence of Ag2O NPs on surface of SF. X-ray diffraction (XRD) analysis revealed the crystalline nature of both SF and Ag2O-SF. Fourier transform infrared spectroscopy (FT-IR) results showed the different forms of silk (I and II) and their corresponding protein (amide I, II, III) confirmations. Biodegradation study revealed insignificant changes in the morphology of Ag2O-SF spuns even after 14 days of immersion in phosphate buffered saline (PBS). Ag2O-SF spuns showed excellent antibacterial activity against both pathogen (S. aureus and M. tuberculosis) and non-pathogen (E. coli) bacteria. More importantly, In vitro wound healing (scratch assay) assay revealed fast migration of the T3T fibroblast cells through the scratch area treated with extract of Ag2O-SF spuns and the area was completely covered within 24 h. Cytotoxicity assay confirmed the biocompatible nature of the Ag2O-SF spuns, thus suggesting an ideal material for wound healing and anti-bacterial applications.

Item Type: Journal Article
Publication: Journal of Colloid and Interface Science
Publisher: Academic Press Inc.
Additional Information: The copyright for this article belongs to the Academic Press Inc.
Keywords: Biocompatibility; Biodegradation; Biological materials; Cell culture; Differential scanning calorimetry; Fourier transform infrared spectroscopy; Oxides; Scanning electron microscopy; Silver nanoparticles; Surface plasmon resonance; X ray diffraction analysis, Anti-bacterial activity; Biodegradation studies; Cytotoxicity assays; Fourier transform infra red (FTIR) spectroscopy; Phosphate-buffered salines; Silk fibroin; Silver oxide nanoparticles; Wound healing, Silver oxides, antibiotic agent; silk fibroin; silver nanoparticle; silver oxide nanoparticle; unclassified drug; wound healing promoting agent; antiinfective agent; disilver oxide; fibroin; metal nanoparticle; oxide; silver derivative, antibacterial activity; Article; biocompatibility; biodegradation; cell migration; cell proliferation; controlled study; crystallization; differential scanning calorimetry; drug potentiation; drug synthesis; Escherichia coli; fibroblast; in vitro study; infrared spectroscopy; Mycobacterium tuberculosis; nanotoxicology; nonhuman; photometry; priority journal; scanning electron microscopy; Staphylococcus aureus; surface plasmon resonance; surface property; ultraviolet spectrophotometry; wound healing; wound healing assay; X ray diffraction; animal; bacterium; chemistry; drug effect; microwave radiation; mouse; NIH 3T3 cell line; wound healing, Animals; Anti-Bacterial Agents; Bacteria; Cell Proliferation; Fibroins; Metal Nanoparticles; Mice; Microwaves; NIH 3T3 Cells; Oxides; Silver Compounds; Wound Healing
Department/Centre: Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited: 10 Aug 2022 05:04
Last Modified: 10 Aug 2022 05:04
URI: https://eprints.iisc.ac.in/id/eprint/75648

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