Isolation and purification of fucoidan from Sargassum ilicifolium: Osteogenic differentiation potential in mesenchymal stem cells for bone tissue engineering

Devi G V, Y and Nagendra, AH and Shenoy  P, S and Chatterjee, K and Venkatesan, J (2022) Isolation and purification of fucoidan from Sargassum ilicifolium: Osteogenic differentiation potential in mesenchymal stem cells for bone tissue engineering. In: Journal of the Taiwan Institute of Chemical Engineers, 136 .

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Abstract

Background: Naturally derived polymeric substances have recently gained much attention in tissue engineering applications due to their unique biological activities. The present work deals with fucoidan's isolation, characterizations, and osteogenic differentiation. The fucoidan has been used for several biological and biomedical applications. The research on fucoidan concerning its application mainly in osteogenic differentiation is scanty. Thus, the study examines the osteogenic differentiation property of purified fucoidan from Sargassum ilicifolium. Methods: The isolation of fucoidan was performed by using probe-sonication combined with microwave extraction and hot water extraction methods. The probe sonication-microwave procedure provides a 1.5X higher yield than the hot water extraction method. The probe sonication-microwave derived crude fucoidan was further purified with DEAE-Cellulose anion-exchange chromatography. The structural characterization of purified fucoidan was carried out by UV–Visible spectroscopy, X-Ray diffraction, Fourier transform infrared spectroscopy, Thermogravimetric analysis, and 1HNMR analysis. In addition, the osteogenic differentiation ability was studied by performing cell proliferation, alkaline phosphatase activity, mineralization, and expression of osteoblast markers with murine mesenchymal stem cells (C3H10T1/2). Significant findings: The findings of the structural study confirm the presence of sulfates and hydroxyl groups in the isolated fucoidan. The 1HNMR results confirm the occurrence of fucose residues. The in vitro study results demonstrate that the purified fucoidan significantly scavenges the formation of free radicles and induces the alkaline phosphatase activity, mineralization, and the expression of osteoblast-specific genes. The findings indicate that the fucoidan from Sargassum ilicifolium can be a potential biomaterial for bone regeneration.

Item Type:	Journal Article
Publication:	Journal of the Taiwan Institute of Chemical Engineers
Publisher:	Taiwan Institute of Chemical Engineers
Additional Information:	The copyright for this article belongs to the Taiwan Institute of Chemical Engineers.
Keywords:	Bone; Cell culture; Cell proliferation; Extraction; Fourier transform infrared spectroscopy; Medical applications; Mineralogy; Phosphatases; Purification; Stem cells; Sulfur compounds; Tissue engineering, 1H NMR; Alkaline phosphatase activity; Fucoidans; Hot water extraction; Mesenchymal stem cell; Mineralisation; Osteogenic differentiation; Probe sonication; Sargassa ilicifolium; Water extraction methods, Thermogravimetric analysis, Cells; Differentiation; Engineering; Extraction; Gravimetry; Phosphatase; Purification; Sulfur Compounds; Thermal Analysis
Department/Centre:	Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited:	15 Jul 2022 10:52
Last Modified:	15 Jul 2022 10:52
URI:	https://eprints.iisc.ac.in/id/eprint/74447

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