Targeted nanoformulation of C1 inhibits the growth of KB spheroids and cancer stem cell-enriched MCF-7 mammospheres

Pradhan, A and Mishra, S and Basu, SM and Surolia, A and Giri, J and Srivastava, R and Panda, D (2021) Targeted nanoformulation of C1 inhibits the growth of KB spheroids and cancer stem cell-enriched MCF-7 mammospheres. In: Colloids and Surfaces B: Biointerfaces, 202 .

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Abstract

C1, a synthetic analog of curcumin, has been reported to show potent antiproliferative effects against a variety of cancer cells. Here, we report a strong anticancer activity of the folate receptor-targeted lipid nanoparticle formulation of C1 against cancer cells and cancer stem cells both in 2D culture and 3D spheroids. The size of the C1 encapsulated folic acid functionalized nanoliposomes (Lipos-C1) was determined to be 83 ± 17 nm. Lipos-C1 nanoparticles displayed sustained C1 release kinetics at both pH 7.4 and 5.5. The folate receptor (FR) targeted nanoliposomes were internalized into FR-positive KB cells via the folate receptor-mediated endocytosis process. Lipos-C1 killed KB cells much more efficiently than C1. Lipos-C1 depolymerized microtubules, generated ROS, caused DNA damage, and induced apoptosis in KB cells. Importantly, Lipos-C1 strongly inhibited the growth of the 3D KB spheroids than C1. Interestingly, Lipos-C1 also suppressed cancer stem cells (CSCs) enriched MCF-7 mammosphere growth by impeding breast cancer stem cells (BCSCs) enrichment, growth, and proliferation. The results suggested that Lipos-C1 could be a promising nanoformulation for cancer chemotherapy. © 2021 Elsevier B.V.

Item Type:	Journal Article
Publication:	Colloids and Surfaces B: Biointerfaces
Publisher:	Elsevier B.V.
Additional Information:	The copyright for this article belongs to Elsevier B.V.
Keywords:	Cell death; Chemotherapy; Drug products; Liposomes; Nanoparticles; Stem cells, 3d spheroid; Cancer cells; Cancer stem cells; Curcumin analog; Folate receptor; Folate-receptor targeted liposome; Mammospheres; Microtubules; Nanoformulation; Nanoliposomes, Diseases
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	02 Jul 2021 16:10
Last Modified:	02 Jul 2021 16:10
URI:	http://eprints.iisc.ac.in/id/eprint/68705

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