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Use of a molecular beacon based fluorescent method for assaying uracil DNA glycosylase (Ung) activity and inhibitor screening

Mehta, A and Raj, P and Sundriyal, S and Gopal, B and Varshney, U (2021) Use of a molecular beacon based fluorescent method for assaying uracil DNA glycosylase (Ung) activity and inhibitor screening. In: Biochemistry and Biophysics Reports, 26 .

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Official URL: https://dx.doi.org/10.1016/j.bbrep.2021.100954


Uracil DNA glycosylases are an important class of enzymes that hydrolyze the N-glycosidic bond between the uracil base and the deoxyribose sugar to initiate uracil excision repair. Uracil may arise in DNA either because of its direct incorporation (against A in the template) or because of cytosine deamination. Mycobacteria with G, C rich genomes are inherently at high risk of cytosine deamination. Uracil DNA glycosylase activity is thus important for the survival of mycobacteria. A limitation in evaluating the druggability of this enzyme, however, is the absence of a rapid assay to evaluate catalytic activity that can be scaled for medium to high-throughput screening of inhibitors. Here we report a fluorescence-based method to assay uracil DNA glycosylase activity. A hairpin DNA oligomer with a fluorophore at its 5� end and a quencher at its 3� ends was designed incorporating five consecutive U:A base pairs immediately after the first base pair (5� C:G 3�) at the top of the hairpin stem. Enzyme assays performed using this fluorescent substrate were seen to be highly sensitive thus enabling investigation of the real time kinetics of uracil excision. Here we present data that demonstrate the feasibility of using this assay to screen for inhibitors of Mycobacterium tuberculosis uracil DNA glycosylase. We note that this assay is suitable for high-throughput screening of compound libraries for uracil DNA glycosylase inhibitors. © 2021

Item Type: Journal Article
Publication: Biochemistry and Biophysics Reports
Publisher: Elsevier B.V.
Additional Information: The copyright of this article belongs to Elsevier B.V.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 05 Mar 2021 09:53
Last Modified: 05 Mar 2021 09:53
URI: http://eprints.iisc.ac.in/id/eprint/68079

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