Biofilms of the non-tuberculous Mycobacterium chelonae form an extracellular matrix and display distinct expression patterns

Vega-Dominguez, P and Peterson, E and Pan, M and Di Maio, A and Singh, S and Umapathy, S and Saini, DK and Baliga, N and Bhatt, A (2020) Biofilms of the non-tuberculous Mycobacterium chelonae form an extracellular matrix and display distinct expression patterns. In: Cell Surface, 6 .

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Abstract

Mycobacterium chelonae is an environmental, non-tuberculous mycobacterial species, capable of causing infections in humans. Biofilm formation is a key strategy used by M. chelonae in colonising niches in the environment and in the host. We studied a water-air interface (pellicle) biofilm of M. chelonae using a wide array of approaches to outline the molecular structure and composition of the biofilm. Scanning electron micrographs showed that M. chelonae biofilms produced an extracellular matrix. Using a combination of biochemical analysis, Raman spectroscopy, and fluorescence microscopy, we showed the matrix to consist of proteins, carbohydrates, lipids and eDNA. Glucose was the predominant sugar present in the biofilm matrix, and its relative abundance decreased in late (established) biofilms. RNA-seq analysis of the biofilms showed upregulation of genes involved in redox metabolism. Additionally, genes involved in mycolic acid, other lipid and glyoxylate metabolism were also upregulated in the early biofilms. © 2020 The Authors

Item Type:	Journal Article
Publication:	Cell Surface
Publisher:	Elsevier B.V.
Additional Information:	Copyright to this article belongs to Elsevier B.V.
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited:	30 Jul 2021 11:41
Last Modified:	30 Jul 2021 11:41
URI:	http://eprints.iisc.ac.in/id/eprint/66346

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