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Structure specific recognition of telomeric repeats containing RNA by the RGG-box of hnRNPA1

Ghosh, M and Singh, M (2020) Structure specific recognition of telomeric repeats containing RNA by the RGG-box of hnRNPA1. In: Nucleic acids research, 48 (8). pp. 4492-4506.

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Official URL: https://dx.doi.org/10.1093/nar/gkaa134


The telomere repeats containing RNA (TERRA) is transcribed from the C-rich strand of telomere DNA and comprises of UUAGGG nucleotides repeats in humans. The TERRA RNA repeats can exist in single stranded, RNA-DNA hybrid and G-quadruplex forms in the cell. Interaction of TERRA RNA with hnRNPA1 has been proposed to play critical roles in maintenance of telomere DNA. hnRNPA1 contains an N-terminal UP1 domain followed by an RGG-box containing C-terminal region. RGG-motifs are emerging as key protein motifs that recognize the higher order nucleic acid structures as well as are known to promote liquid-liquid phase separation of proteins. In this study, we have shown that the RGG-box of hnRNPA1 specifically recognizes the TERRA RNA G-quadruplexes that have loops in their topology, whereas it does not interact with the single-stranded RNA. Our results show that the N-terminal UP1 domain in the presence of the RGG-box destabilizes the loop containing TERRA RNA G-quadruplex efficiently compared to the RNA G-quadruplex that lacks loops, suggesting that unfolding of G-quadruplex structures by UP1 is structure dependent. Furthermore, we have compared the telomere DNA and TERRA RNA G-quadruplex binding by the RGG-box of hnRNPA1 and discussed its implications in telomere DNA maintenance. .

Item Type: Journal Article
Publication: Nucleic acids research
Publisher: NLM (Medline)
Additional Information: copy right for this article belongs to NLM (Medline)
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility)
Date Deposited: 09 Oct 2020 10:21
Last Modified: 09 Oct 2020 10:21
URI: http://eprints.iisc.ac.in/id/eprint/65421

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