Ghosh, M and Singh, M (2020) Structure specific recognition of telomeric repeats containing RNA by the RGG-box of hnRNPA1. In: Nucleic acids research, 48 (8). pp. 4492-4506.
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Abstract
The telomere repeats containing RNA (TERRA) is transcribed from the C-rich strand of telomere DNA and comprises of UUAGGG nucleotides repeats in humans. The TERRA RNA repeats can exist in single stranded, RNA-DNA hybrid and G-quadruplex forms in the cell. Interaction of TERRA RNA with hnRNPA1 has been proposed to play critical roles in maintenance of telomere DNA. hnRNPA1 contains an N-terminal UP1 domain followed by an RGG-box containing C-terminal region. RGG-motifs are emerging as key protein motifs that recognize the higher order nucleic acid structures as well as are known to promote liquid-liquid phase separation of proteins. In this study, we have shown that the RGG-box of hnRNPA1 specifically recognizes the TERRA RNA G-quadruplexes that have loops in their topology, whereas it does not interact with the single-stranded RNA. Our results show that the N-terminal UP1 domain in the presence of the RGG-box destabilizes the loop containing TERRA RNA G-quadruplex efficiently compared to the RNA G-quadruplex that lacks loops, suggesting that unfolding of G-quadruplex structures by UP1 is structure dependent. Furthermore, we have compared the telomere DNA and TERRA RNA G-quadruplex binding by the RGG-box of hnRNPA1 and discussed its implications in telomere DNA maintenance. .
| Item Type: | Journal Article |
|---|---|
| Publication: | Nucleic acids research |
| Publisher: | NLM (Medline) |
| Additional Information: | copy right for this article belongs to NLM (Medline) |
| Department/Centre: | Division of Biological Sciences > Molecular Biophysics Unit Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility) |
| Date Deposited: | 09 Oct 2020 10:21 |
| Last Modified: | 09 Oct 2020 10:21 |
| URI: | http://eprints.iisc.ac.in/id/eprint/65421 |
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