Real Time Measurement of Deformability Index for Electro-Mechanodiagnostics

Kotesa, R S and Rattan, K and Ananthasuresh, G K and Sen, P (2018) Real Time Measurement of Deformability Index for Electro-Mechanodiagnostics. In: Proceedings of the 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, 22-26 April, 2018, Singapore, pp. 189-192.

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Abstract

We report a novel microfluidic platform to measure the stiffness of a single cell by evaluating its deformability index through the time information embedded in the electrical impedance data of the cell transit. The deformability index and transit time are two parameters that quantify the stiffness of a single cell passing through a constriction. Conventionally, the deformability index has been measured optically using a high-speed camera, which is expensive, bulky, time-consuming, and thus limits the portability and ease of mechanodiagnostics. Furthermore, the transit time of cell passage is prone to inadvertent flow rate fluctuations. We use Electrical Impedance Spectroscopy combined with a microfluidic channel integrated with an array of coplanar electrodes to evaluate the deformability index of the biological cells. Additionally, measurements of cell impedance during cell transit provides a multi-modal electromechanical signature of individual cells that can be correlated to their health or disease states. The portable and non-invasive method of mechano-phenotyping cell population would make electro-mechanodiagnostics possible.

Item Type:	Conference Paper
Series.:	International Conference on Nano-Micro Engineered and Molecular Systems NEMS
Publisher:	IEEE
Additional Information:	13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS), Singapore, SINGAPORE, APR 22-26, 2018
Keywords:	Cell Mechanics; Electrical Impedance Spectroscopy; Deformability Cytometry; Single cell; Microfluidics
Department/Centre:	Division of Mechanical Sciences > Mechanical Engineering Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited:	26 Jun 2019 17:12
Last Modified:	26 Jun 2019 17:12
URI:	http://eprints.iisc.ac.in/id/eprint/62814

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