Rajesh, Rajaian P and Franklin, Jayaseelan B (2018) Identification of Conotoxins with Novel Odd Number of Cysteine Residues from the Venom of a Marine Predatory Gastropod Conus leopardus Found in Andaman Sea. In: PROTEIN AND PEPTIDE LETTERS, 25 (11). pp. 1035-1040.
Full text not available from this repository.Abstract
Background: Conotoxins are neuro-pharmacologically active cysteine rich peptides isolated from the venom complex of marine cone snails. These are usually made of even number of cysteines. Method: In this study we characterised six novel conotoxin sequences from the venom of Conus leopardus collected from the Andaman Sea, namely Le907 (C-C), Le868 (C-C), Le933 (-C-CC), Le949 (-C-CC), Le1988 (C-C-CC-C) and Le1642 (CC-C-C) using de novo mass spectrometry-based sequencing methods. Astonishingly 3 of these peptides possess novel arrangements of cysteine residues with odd number of cysteines (-C-CC; C-C-CC-C), namely Le933, Le949 and Le1988. Further, a post-translational variant of peptide Le933 was identified and experimentally determined to contain hydroxyproline. Results: The unusual cysteine arrangements observed suggests novel class of conotoxins. These results expand our understanding of the diversity of odd cysteine arrangements in conotoxins.
| Item Type: | Journal Article |
|---|---|
| Publication: | PROTEIN AND PEPTIDE LETTERS |
| Publisher: | BENTHAM SCIENCE PUBL LTD |
| Additional Information: | Copyright of this article belongs to BENTHAM SCIENCE PUBL LTD |
| Keywords: | Cone snail; Corms leopardus; conotoxins; odd cysteines; novel cysteine arrangement; mass spectrometry |
| Department/Centre: | Division of Biological Sciences > Molecular Biophysics Unit |
| Date Deposited: | 28 Jan 2019 10:13 |
| Last Modified: | 28 Jan 2019 10:13 |
| URI: | http://eprints.iisc.ac.in/id/eprint/61508 |
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