Ultra-Sensitive Detection of Proteins Using Chemically Modified Nanoporous PVDF Membrane with Attenuated Near IR Autofluorescence

Padmavathy, Nagarajan and Das Ghosh, Lopamudra and Sharma, Maya and Remanan, Sanjay and Chatterjee, Kaushik and Bose, Suryasarathi (2018) Ultra-Sensitive Detection of Proteins Using Chemically Modified Nanoporous PVDF Membrane with Attenuated Near IR Autofluorescence. In: CHEMISTRYSELECT, 3 (13). pp. 3839-3847.

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Abstract

We report the lowest autofluorescent nanoporous polyvinylidene fluoride (PVDF) membranes exhibit near infra-red (NIR) emission properties for western blot detection of high and low molecular weight proteins. The design involves post modification of PVDF membranes by an alkali treatment that reduces the native of PVDF at 450-520nm. The background fluorescence of these modified membranes is eight times lower than the commercial available PVDF membranes and displayed NIR emission at 750nm. Imparted alkene conjugated double bonds in the polymeric backbone by alkali treatment causes the NIR emission in the modified PVDF membranes and this translates improvement in detection of, in particular, high molecular weight proteins (130 kDa) compared to traditional western blot. To validate the pore size effect, two different pores sized (similar to 100nm and similar to 0.8m) PVDF membranes were prepared, surface modified and subjected for protein profiling. High linearity was achieved in detection of high molecular weight proteins and significant protein binding was noticed for the alkali treated membranes of similar to similar to 100nm size. The methodology permits the design of modified PVDF membranes with lesser pore size could be an alternative for existing membranes with minimal autofluorescence for efficient and quick detection of high molecular weight proteins.

Item Type:	Journal Article
Publication:	CHEMISTRYSELECT
Publisher:	Wiley-Blackwell
Additional Information:	The Copyright for this article belong to Wiley-Blackwell.
Keywords:	alkali treatment; autofluorescence; nano porous PVDF membrane; NIR emission; western blot
Department/Centre:	Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited:	04 May 2018 18:48
Last Modified:	02 Aug 2022 10:41
URI:	https://eprints.iisc.ac.in/id/eprint/59730

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