Mass spectrometric identification of bromotryptophan containing conotoxin sequences from the venom of C. amadis

Vijayasarathy, M and Balaram, P (2018) Mass spectrometric identification of bromotryptophan containing conotoxin sequences from the venom of C. amadis. In: TOXICON, 144 . pp. 68-74.

PDF Tax_144_68_2018.pdf - Published Version Restricted to Registered users only Download (2MB) | Request a copy

Abstract

Four 30 residue conotoxin have been identified from the venom of C. amadis. MS/MS analysis of crude venom subjected to global reduction/alkylation yielded fragmentation patterns, which permitted searching and matching with a database of putative mature toxin sequences obtained from transcriptomic analysis. Of the four sequences identified, Am3408(Am6.1b), Am3452(Am6.1c), Am3136(Am6.2a) and Am3214(Am6.2b), three contain bromotryptophan residues, while an additional post translational modification, gamma carboxylation of glutamic acid, is present in Am3408(Am6.lb)/3452(Am6.1c). The conotoxins belong to the O1/O2 gene superfamily and possess cysteine framework VI/VII. While, the cysteine patterns show a similarity to omega conotoxins, the three C. amadis peptides are highly negatively charged and possess a significant content of hydrophobic residues. (C) 2018 Published by Elsevier Ltd.

Item Type:	Journal Article
Publication:	TOXICON
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
Additional Information:	Copy right for this article belong to PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	16 Apr 2018 20:09
Last Modified:	16 Apr 2018 20:09
URI:	http://eprints.iisc.ac.in/id/eprint/59588

Actions (login required)

View Item