Synthetic arabinomannan glycolipids impede mycobacterial growth, sliding motility and biofilm structure

Syal, Kirtimaan and Maiti, Krishnagopal and Naresh, Kottari and Avaji, Prakash Gouda and Chatterji, Dipankar and Jayaraman, Narayanaswamy (2016) Synthetic arabinomannan glycolipids impede mycobacterial growth, sliding motility and biofilm structure. In: GLYCOCONJUGATE JOURNAL, 33 (5). pp. 763-777.

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Abstract

Mycobacterium has evolved distinct cell wall and strategies such as biofilm formation, which helps it to survive in hostile conditions. We have reported previously that arabinofuranoside containing glycolipids exhibit inhibition activities against the above functions of the mycobacterial species M. smegmatis. In search for activities mediated by oligosaccharide glycolipids, we report herein the inhibitory activities of a linear and a branched pentasaccharides having arabinan and mannan moieties. In the presence of the pentasaccharide glycolipids, a significant reduction in mycobacterial growth is observed, concomitant with reductions in sliding motility and colonization through biofilm formation, at the optimal glycolipid concentrations of 50-100 mu g mL(-1). Especially the biofilm coat is ruptured by similar to 80-85 % in the presence of glycolipids. Pentasaccharides alone without the lipidic chain show only a weak effect. The glycolipids are non-toxic, as evaluated through their effect on RBCs. Analysis of the mycolic acid profile of glycolipid treated biofilm shows that a-and epoxy mycolic acids are downregulated significantly, in comparison to glycolipid untreated biofilms. Lipidomics profile analysis through mass spectrometry further reveals profound downregulation of phosphatidylinositol mannosides, acylatedphosphoglycerols and mycolic acid family, namely, keto-, alpha- and methoxymycolic acids.

Item Type:	Journal Article
Publication:	GLYCOCONJUGATE JOURNAL
Additional Information:	Copy right for this article belongs to the SPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit Division of Chemical Sciences > Organic Chemistry
Date Deposited:	03 Dec 2016 05:17
Last Modified:	03 Dec 2016 05:17
URI:	http://eprints.iisc.ac.in/id/eprint/55241

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