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Rapid identification of amino acid types in proteins using phase modulated 2D HN(CACB) and 2D HN(COCACB)

Dubey, Abhinav and Mondal, Somnath and Chandra, Kousik and Atreya, Hanudatta S (2016) Rapid identification of amino acid types in proteins using phase modulated 2D HN(CACB) and 2D HN(COCACB). In: JOURNAL OF MAGNETIC RESONANCE, 267 . pp. 22-29.

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Official URL: http://dx.doi.org/10.1016/j.jmr.2016.04.004


We present a simple approach to rapidly identify amino acid types in proteins from a 2D spectrum. The method is based on the fact that C-13 beta chemical shifts of different amino acid types fall in distinct spectral regions. By evolving the C-13 chemical shifts in the conventional HNCACB or HN(CO)CACB type experiment for a single specified delay period, the phase of the cross peaks of different amino acid residues are modulated depending on their C-13 beta shift values. Following this specified evolution period, the 2D HN projections of these experiments are acquired. The C-13 evolution period can be chosen such that all residues belonging to a given set of amino acid types have the same phase pattern (positive or negative) facilitating their identification. This approach does not require the preparation of any additional samples, involves the analysis of 2D N-15-H-1] HSQC-type spectra obtained from the routinely used triple resonance experiments with minor modifications, and is applicable to deuterated proteins. The method will be useful for quick assignment of signals that shift during ligand binding or in combination with selective labeling/unlabeling approaches for identification of amino acid types to aid the sequential assignment process. (C) 2016 Elsevier Inc. All rights reserved.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
Keywords: Amino acid-type assignments; Sequential assignments; Protein structure
Department/Centre: Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility)
Division of Chemical Sciences > Solid State & Structural Chemistry Unit
Date Deposited: 16 Jun 2016 07:44
Last Modified: 16 Jun 2016 07:44
URI: http://eprints.iisc.ac.in/id/eprint/53995

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