Structural characterization and in vitro biomedical activities of sulfated chitosan from Sepia pharaonis

Karthik, Ramachandran and Manigandan, Venkatesan and Saravanan, Ramachandran and Rajesh, Rajaian Pushpabai and Chandrika, Baby (2016) Structural characterization and in vitro biomedical activities of sulfated chitosan from Sepia pharaonis. In: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 84 . pp. 319-328.

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Abstract

A low molecular weight sulfated chitosan (SP-LMWSC) was isolated from the cuttlebone of Sepia pharaonis. Elemental analysis established the presence of C, H and N. The sulfation of SP-LMWSC was confirmed by the presence of characteristic peaks in FT-IR and FT-Raman spectra. The thermal properties of SP-LMWSC were studied by thermogravimetric analysis and differential scanning calorimetry. Electrolytic conductivity of SP-LMWSC was measured by cyclic voltammetry and the molecular weight was determined by MALDI-TOF/MS. The molecular structure and sulfation sites of SP-LMWSC were unambiguously confirmed using H-1,C-13, 2D COSY and 2D HSQC NMR spectroscopy. SP-LMWSC exhibited increased anticoagulant activity in avian blood by delaying coagulation parameters and displayed cytostatic activity by inhibiting the migration of avian leucocytes. SP-LMWSC demonstrated avian antiviral activity by binding to Newcastle disease virus receptors at a low titer value of 1/64. These findings suggested that SP-LMWSC isolated from an industrial discard holds immense potentials as carbohydrate based pharmaceuticals in future. (C) 2015 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Publication:	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Publisher:	ELSEVIER SCIENCE BV
Additional Information:	Copy right for this article belongs to the ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
Keywords:	Sulfated chitosan; Structural characterization; Avian biomedical application
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	03 Mar 2016 07:35
Last Modified:	03 Mar 2016 07:35
URI:	http://eprints.iisc.ac.in/id/eprint/53346

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