Crystallographic studies of the extracytoplasmic function sigma factor sigma(J) from Mycobacterium tuberculosis

Goutam, Kapil and Gupta, Arvind Kumar and Gopal, Balasubramanian (2015) Crystallographic studies of the extracytoplasmic function sigma factor sigma(J) from Mycobacterium tuberculosis. In: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 71 (8). pp. 946-950.

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Abstract

Mycobacterium tuberculosis has multiple sigma factors which enable the bacterium to reprogram its transcriptional machinery under diverse environmental conditions. sigma(J), an extracytoplasmic function sigma factor, is upregulated in late stationary phase cultures and during human macrophage infection. sigma(J) governs the cellular response to hydrogen peroxide-mediated oxidative stress. sigma(J) differs from other canonical sigma factors owing to the presence of a SnoaL_2 domain at the C-terminus. sigma(J) crystals belonged to the tetragonal space group I422, with unit-cell parameters a = b = 133.85, c = 75.08 angstrom. Diffraction data were collected to 2.16 angstrom resolution on the BM14 beamline at the European Synchrotron Radiation Facility (ESRF).

Item Type:	Journal Article
Publication:	ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
Publisher:	INT UNION CRYSTALLOGRAPHY
Additional Information:	Copy right for this article belongs to the INT UNION CRYSTALLOGRAPHY, 2 ABBEY SQ, CHESTER, CH1 2HU, ENGLAND
Keywords:	sigma factor; sigma factor; SnoaL_2 domain; potential drug target
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	11 Sep 2015 04:53
Last Modified:	11 Sep 2015 04:53
URI:	http://eprints.iisc.ac.in/id/eprint/52329

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