Characterization of a dual-active enzyme, DcpA, involved in cyclic diguanosine monophosphate turnover in Mycobacterium smegmatis

Sharma, Indra Mani and Prakash, Sunita and Dhanaraman, Thillaivillalan and Chatterji, Dipankar (2014) Characterization of a dual-active enzyme, DcpA, involved in cyclic diguanosine monophosphate turnover in Mycobacterium smegmatis. In: MICROBIOLOGY-SGM, 160 (10). pp. 2304-2318.

PDF mic_sgm_160_2304_2014.pdf - Published Version Restricted to Registered users only Download (1MB) | Request a copy

Abstract

We have reported previously that the long-term survival of Mycobacterium smegmatis is facilitated by a dual-active enzyme MSDGC-1 (renamed DcpA), which controls the cellular turnover of cyclic diguanosine monophosphate (c-di-GMP). Most mycobacterial species possess at least a single copy of a DcpA orthologue that is highly conserved in terms of sequence similarity and domain architecture. Here, we show that DcpA exists in monomeric and dimeric forms. The dimerization of DcpA is due to non-covalent interactions between two protomers that are arranged in a parallel orientation. The dimer shows both synthesis and hydrolysis activities, whereas the monomer shows only hydrolysis activity. In addition, we have shown that DcpA is associated with the cytoplasmic membrane and exhibits heterogeneous cellular localization with a predominance at the cell poles. Finally, we have also shown that DcpA is involved in the change in cell length and colony morphology of M. smegmatis. Taken together, our study provides additional evidence about the role of the bifunctional protein involved in c-di-GMP signalling in M. smegmatis.

Item Type:	Journal Article
Publication:	MICROBIOLOGY-SGM
Publisher:	SOC GENERAL MICROBIOLOGY
Additional Information:	Copyright for this article belongs to the SOC GENERAL MICROBIOLOGY, MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	12 Jan 2015 05:53
Last Modified:	12 Jan 2015 05:53
URI:	http://eprints.iisc.ac.in/id/eprint/50590

Actions (login required)

View Item