Dual role of MsRbpA: transcription activation and rescue of transcription from the inhibitory effect of rifampicin

Verma, Amit Kumar and Chatterji, Dipankar (2014) Dual role of MsRbpA: transcription activation and rescue of transcription from the inhibitory effect of rifampicin. In: MICROBIOLOGY-SGM, 160 (9). pp. 2018-2029.

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Abstract

MsRbpA is an RNA polymerase (RNAP) binding protein from Mycobacterium smegmatis. According to previous studies, MsRbpA rescues rifampicin-induced transcription inhibition upon binding to the RNAP. Others have shown that RbpA from Mycobacterium tuberculosis (MtbRbpA) is a transcription activator. In this study, we report that both MsRbpA and MtbRbpA activate transcription as well as rescue rifampicin-induced transcription inhibition. Transcription activation is achieved through the increased formation of closed RNAP-promoter complex as well as enhanced rate of conversion of this complex to a stable transcriptionally competent RNAP promoter complex. When a 16 aa peptide fragment (Asp 58 to Lys 73) was deleted from MsRbpA, the resulting protein showed 1000-fold reduced binding with core RNAP. The deletion results in abolition of transcription activation and rescue of transcription from the inhibitory effect of rifampicin. Through alanine scanning of this essential region of MsRbpA, Gly 67, Val 69, Pro 70 and Pro 72 residues are identified to be important for MsRbpA function. Furthermore, we report here that the protein is indispensable for M. smegmatis, and it appears to help the organism grow in the presence of the antibiotic rifampicin.

Item Type:	Journal Article
Publication:	MICROBIOLOGY-SGM
Publisher:	SOC GENERAL MICROBIOLOGY
Additional Information:	Copy right for this article belongs to the SOC GENERAL MICROBIOLOGY, MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	04 Dec 2014 04:49
Last Modified:	04 Dec 2014 04:49
URI:	http://eprints.iisc.ac.in/id/eprint/50374

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