Biodegradation of acrylamide by acrylamidase from Stenotrophomonas acidaminiphila MSU12 and analysis of degradation products by MALDI-TOF and HPLC

Lakshmikandan, M and Sivaraman, K and Raja, Elaiya S and Vasanthakumar, P and Rajesh, RP and Sowparthani, K and Jebasingh, Emmanuel Joshua S (2014) Biodegradation of acrylamide by acrylamidase from Stenotrophomonas acidaminiphila MSU12 and analysis of degradation products by MALDI-TOF and HPLC. In: INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 94 . pp. 214-221.

PDF int_bio_bio_94_214_2014.pdf - Published Version Restricted to Registered users only Download (1MB) | Request a copy

Abstract

The present study examines an improved detoxification and rapid biological degradation of toxic pollutant acrylamide using a bacterium. The acrylamide degrading bacterium was isolated from the soil followed by its screening to know the acrylamide degrading capability. The minimal medium containing acrylamide (30 mM) served as a sole source of carbon and nitrogen for their active growth. The optimization of three different factors was analyzed by using Response Surface Methodology (RSM). The bacteria actively degraded the acrylamide at a temperature of 32 degrees C, with a maximum growth at 30 mM substrate (acrylamide) concentration at a pH of 7.2. The acrylamidase activity and degradation of acrylamide was determined by High Performance Liquid Chromatography (HPLC) and Matrix Assisted Laser Desorption and Ionization Time of Flight mass spectrometer (MALDI-TOF). Based on 168 rRNA analysis the selected strain was identified as Gram negative bacilli Stenotrophomonas acidaminiphila MSU12. The acrylamidase was isolated from bacterial extract and was purified by HPLC, whose mass spectrum showed a molecular mass of 38 kDa. (C) 2014 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Publication:	INTERNATIONAL BIODETERIORATION & BIODEGRADATION
Publisher:	ELSEVIER SCI LTD
Additional Information:	Copy right for this article belongs to the ELSEVIER SCI LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
Keywords:	Acrylamidase; Acrylamide; Biodegradation; MALDI-TOF; Stenotrophomonas acidaminiphila MSU12
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	04 Dec 2014 04:45
Last Modified:	04 Dec 2014 04:45
URI:	http://eprints.iisc.ac.in/id/eprint/50339

Actions (login required)

View Item