Evaluation of hexane and ethyl acetate extracts of the sponge Jaspis diastra collected from Mauritius Waters on HeLa cells

Beedessee, Girish and Ramanjooloo, Avin and Tiscornia, Ines and Cresteil, Thierry and Raghothama, Srinivasarao and Arya, Deepak and Rao, Shashanka and Gowd, Konkallu Hanumae and Bollati-Fogolin, Mariela and Marie, Daniel EP (2014) Evaluation of hexane and ethyl acetate extracts of the sponge Jaspis diastra collected from Mauritius Waters on HeLa cells. In: JOURNAL OF PHARMACY AND PHARMACOLOGY, 66 (9). pp. 1317-1327.

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Abstract

Objectives Based on previous screening results, the cytotoxic effect of the hexane (JDH) and ethyl acetate extracts (JDE) of the marine sponge Jaspis diastra were evaluated on HeLa cells and the present study aimed at determining their possible mechanism of cell death. Methods Nuclear staining, membrane potential change, flow cytometry analysis of cell cycle distribution and annexin V staining were undertaken to investigate the effects of JDE and JDH. Electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance were used to characterize an isolated bioactive molecule. Key findings JDE displayed an IC50 25 times more significant than the JDH. Flow cytometry analysis revealed JDE induced apoptosis in HeLa cells accompanied by the collapse of mitochondrial membrane potential. Fractionation of JDE resulted in the isolation of the known cytotoxic cyclodepsipeptide, Jaspamide. Conclusions Taking our results together suggest that JDE can be valuable for the development of anticancer drugs, especially for cervical cancer. Further investigations are currently in progress with the aim to determine and isolate other bioactive compounds from this extract.

Item Type:	Journal Article
Publication:	JOURNAL OF PHARMACY AND PHARMACOLOGY
Publisher:	WILEY-BLACKWELL
Additional Information:	Copy right for this article belongs to the WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
Keywords:	cytotoxic; HeLa cells; Jaspis diastra; Mauritius; sponges
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility)
Date Deposited:	24 Sep 2014 05:49
Last Modified:	24 Sep 2014 05:49
URI:	http://eprints.iisc.ac.in/id/eprint/49924

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