Titania nanotube-modified screen printed carbon electrodes enhance the sensitivity in the electrochemical detection of proteins

Mandal, Soumit S and Navratna, Vikas and Sharma, Pratyush and Gopal, B and Bhattacharyya, Aninda J (2014) Titania nanotube-modified screen printed carbon electrodes enhance the sensitivity in the electrochemical detection of proteins. In: BIOELECTROCHEMISTRY, 98 . pp. 46-52.

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Abstract

The use of titania nanotubes (TiO2-NT) as the working electrode provides a substantial improvement in the electrochemical detection of proteins. A biosensor designed using this strategy provided a robust method to detect protein samples at very low concentrations (C-protein ca 1 ng/mu l). Reproducible measurements on protein samples at this concentration (I-p,I-a of 80 +/- 1.2 mu A) could be achieved using a sample volume of ca 30 mu l. We demonstrate the feasibility of this strategy for the accurate detection of penicillin binding protein, PBP2a, a marker for methicillin resistant Staphylococcus aureus (MRSA). The selectivity and efficiency of this sensor were also validated using other diverse protein preparations such as a recombinant protein tyrosine phosphatase (PTP10D) and bovine serum albumin (BSA). This electrochemical method also presents a substantial improvement in the time taken (few minutes) when compared to conventional enzyme-linked immunosorbent assay (ELISA) protocols. It is envisaged that this sensor could substantially aid in the rapid diagnosis of bacterial infections in resource strapped environments. (C) 2014 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Publication:	BIOELECTROCHEMISTRY
Publisher:	ELSEVIER SCIENCE SA
Additional Information:	copyright for this article belongs to ELSEVIER SCIENCE SA, PO BOX 564, 1001 LAUSANNE, SWITZERLAND
Keywords:	Electrochemical biosensor; Penicillin binding protein 2a; Methicillin resistant Staphylococcus aureus; Titania nanotubes
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit Division of Chemical Sciences > Solid State & Structural Chemistry Unit
Date Deposited:	16 Jul 2014 08:38
Last Modified:	16 Jul 2014 08:38
URI:	http://eprints.iisc.ac.in/id/eprint/49401

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