Evaluation of the role of sigma B in Mycobacterium smegmatis

Mukherjee, Raju and Chatterji, Dipankar (2005) Evaluation of the role of sigma B in Mycobacterium smegmatis. In: Biochemical and Biophysical Research Communications, 338 (2). pp. 964-972.

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Abstract

The alternate sigma factor, sigB, is known to play a crucial role in maintaining the stationary phase in mycobacteria. In this communication, we have studied the proteomics of Mycobacterium smegmatis $mc^2155$ and its two derivatives, one of which has a disrupted sigB gene and the other, PMVSigB, which contains a multicopy plasmid containing sigB. We have identi.ed by two-dimensional gel analyses, several proteins that are over-expressed in PMVSigB compared to $mc^2155$. These proteins are either stress proteins or participate actively in di.erent metabolic pathways of the organisms. On the other hand, when sigB deleted mycobacteria were grown until the stationary phase and its two-dimensional protein pro.le was compared to that of $mc^2155$, few DNA binding proteins were found to be up-regulated. We have shown recently that upon over-expressing sigB, the cell surface glycopeptidolipids of M. smegmatis are hyperglycosylated, a situation similar to what was observed for nutritionally starved bacteria. Gene expression pro.le through quantitative PCR presented here identi.ed a Rhamnosyltransferase responsible for this hyperglycosylation.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier
Additional Information:	The Copyright belongs to Elsevier.
Keywords:	2-D PAGE;Mycobacterium smegmatis;sigB;Glycosyltransferase
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	06 Jan 2006
Last Modified:	19 Sep 2010 04:22
URI:	http://eprints.iisc.ac.in/id/eprint/4857

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