Biodegradation of acrylamide and purification of acrylamidase from newly isolated bacterium Moraxella osloensis MSU11

Jebasingh, S Emmanuel Joshua and Lakshmikandan, M and Rajesh, RP and Raja, P (2013) Biodegradation of acrylamide and purification of acrylamidase from newly isolated bacterium Moraxella osloensis MSU11. In: INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 85 (SI). pp. 120-125.

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Abstract

The increasing industrial utilization of polyacrylamide to assist water clarification, sludge conditioning, papermaking, and secondary oil recovery leads to environmental pollution. In this work, an acrylamide degrading bacterium was isolated from paper mill effluent at Charan mahadevi, Tamilnadu, India. The minimal medium containing acrylamide (40 mM) served as a sole source of carbon and nitrogen for acrylamide degrading bacteria. The bacterial strain has grown well in 40 mM acrylamide at pH (6-7) at 30 degrees C. Within 24-48 h acrylamide was converted into acrylic acid and other metabolites. Based on biochemical characteristics and 16S rRNA gene sequence, the bacterial strain was identified as Gram negative, diplobacilli Moraxella osloensis MSU11. The acrylamide hydrolyzing bacterial enzyme acrylamidase was purified by HPLC. The enzyme molecular weight was determined to be approximately 38 kDa by SDS-PAGE using reference enzyme Pectinase. These results show that M. osloensis MSU11 has a potential to degrade the acrylamide present in the environment. (C) 2013 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Publication:	INTERNATIONAL BIODETERIORATION & BIODEGRADATION
Publisher:	ELSEVIER SCI LTD
Additional Information:	copyright for this article belongs to ELSEVIER SCI LTD ENGLAND
Keywords:	Acrylamide degradation; Acrylamidase; Biodegradation; Moraxella osloensis MSU11
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	22 Dec 2013 15:00
Last Modified:	22 Dec 2013 15:00
URI:	http://eprints.iisc.ac.in/id/eprint/47958

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