Identification and characterization of starvation induced msdgc-1 promoter involved in the c-di-GMP turnover

Bharati, Binod K and Swetha, RK and Chatterji, Dipankar (2013) Identification and characterization of starvation induced msdgc-1 promoter involved in the c-di-GMP turnover. In: GENE, 528 (2). pp. 99-108.

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Abstract

C-di-GMP Bis-(3'-5')-cyclic-dimeric-guanosine monophosphate], a second messenger is involved in intracellular communication in the bacterial species. As a result several multi-cellular behaviors in both Gram-positive and Gram-negative bacteria are directly linked to the intracellular level of c-di-GMP. The cellular concentration of c-di-GMP is maintained by two opposing activities, diguanylate cyclase (DGC) and phosphodiesterase (PDE-A). In Mycobacterium smegmatis, a single bifunctional protein MSDGC-1 is responsible for the cellular concentration of c-di-GMP. A better understanding of the regulation of c-di-GMP at the genetic level is necessary to control the function of above two activities. In this work, we have characterized the promoter element present in msdgc-1 along with the + 1 transcription start site and identified the sigma factors that regulate the transcription of msdgc-1. Interestingly, msdgc-1 utilizes SigA during the initial phase of growth, whereas near the stationary phase SigB containing RNA polymerase takes over the expression of msdgc-1. We report here that the promoter activity of msdgc-1 increases during starvation or depletion of carbon source like glucose or glycerol. When msdgc-1 is deleted, the numbers of viable cells are similar to 10 times higher in the stationary phase in comparison to that of the wild type. We propose here that msdgc-1 is involved in the regulation of cell population density. (C) 2013 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Publication:	GENE
Publisher:	ELSEVIER SCIENCE BV
Additional Information:	Copyright for this article belongs to Science Direct
Keywords:	msdgc-1 promoter; c-di-GMP regulation; beta-Galactosidase; Sigma factors; Transcription
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	25 Oct 2013 06:50
Last Modified:	25 Oct 2013 06:50
URI:	http://eprints.iisc.ac.in/id/eprint/47563

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