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Does aluminium bind to histidine? an NMR investigation of Amyloid beta 12 and Amyloid beta 16 fragments

Narayan, Priya and Krishnarjuna, Bankala and Vishwanathan, Vinaya and Kumar, Dasappa Jagadeesh and Babu, Sudhir and Ramanathan, Krishna Venkatachala and Easwaran, Kalpathy Ramaier Katchap and Nagendra, Holenarasipur GunduRao and Raghothama, Srinivasarao (2013) Does aluminium bind to histidine? an NMR investigation of Amyloid beta 12 and Amyloid beta 16 fragments. In: Chemical Biology & Drug Design, 82 (1). pp. 48-59.

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Official URL: http://dx.doi.org/10.1111/cbdd.12129


Aluminium and zinc are known to be the major triggering agents for aggregation of amyloid peptides leading to plaque formation in Alzheimer's disease. While zinc binding to histidine in A (amyloid ) fragments has been implicated as responsible for aggregation, not much information is available on the interaction of aluminium with histidine. In the NMR study of the N-terminal A fragments, DAEFRHDSGYEV (A12) and DAEFRHDSGYEVHHQK (A16) presented here, the interactions of the fragments with aluminium have been investigated. Significant chemical shifts were observed for few residues near the C-terminus when aluminium chloride was titrated with A12 and A16 peptides. Surprisingly, it is nonhistidine residues which seem to be involved in aluminium binding. Based on NMR constrained structure obtained by molecular modelling, aluminium-binding pockets in A12 were around charged residues such as Asp, Glu. The results are discussed in terms of native structure propagation, and the relevance of histidine residues in the sequences for metal-binding interactions. We expect that the study of such short amyloid peptide fragments will not only provide clues for plaque formation in aggregated conditions but also facilitate design of potential drugs for these targets.

Item Type: Journal Article
Publication: Chemical Biology & Drug Design
Publisher: Wiley-Blackwell
Additional Information: Copyright of this article belongs to Wiley-Blackwell.
Keywords: Aluminium Interactions; Alzheimer's Disease; Amyloid Peptides; A Peptides; Metal Binding; NMR
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility)
Date Deposited: 03 Sep 2013 06:56
Last Modified: 03 Sep 2013 06:56
URI: http://eprints.iisc.ac.in/id/eprint/46965

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