Severe diffraction anisotropy, rotational pseudosymmetry and twinning complicate the refinement of a pentameric coiled-coil structure of NSP4 of rotavirus

Chacko, Anita R and Zwart, Peter H and Read, Randy J and Dodson, Eleanor J and Rao, CD and Suguna, Kaza (2012) Severe diffraction anisotropy, rotational pseudosymmetry and twinning complicate the refinement of a pentameric coiled-coil structure of NSP4 of rotavirus. In: Acta Crystallographica Section D Biological Crystallography, 68 (11). pp. 1541-1548.

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Abstract

The crystal structure of the region spanning residues 95-146 of the rotavirus nonstructural protein NSP4 from the asymptomatic human strain ST3 was determined at a resolution of 2.5 angstrom. Severe diffraction anisotropy, rotational pseudo-symmetry and twinning complicated the refinement of this structure. A systematic explanation confirming the crystal pathologies and describing how the structure was successfully refined is given in this report.

Item Type:	Journal Article
Publication:	Acta Crystallographica Section D Biological Crystallography
Publisher:	International Union of Crystallography
Additional Information:	Copyright of this article belongs to International Union of Crystallography.
Keywords:	Data Pathology; Diffraction Anisotropy; Rotational Pseudosymmetry; Twinning
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	18 Feb 2013 07:51
Last Modified:	18 Feb 2013 07:51
URI:	http://eprints.iisc.ac.in/id/eprint/45367

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