H-1, C-13, N-15 assignment and secondary structure determination of glutamine amido transferase subunit of gaunosine monophosphate synthetase from Methanocaldococcus jannaschii

Ali, Rustam and Kumar, Sanjeev and Balaram, Hemalatha and Sarma, Siddhartha P (2012) H-1, C-13, N-15 assignment and secondary structure determination of glutamine amido transferase subunit of gaunosine monophosphate synthetase from Methanocaldococcus jannaschii. In: BIOMOLECULAR NMR ASSIGNMENTS, 6 (2). pp. 193-196.

	PDF biomed_nmr_ass_6_193_2012.pdf - Published Version Restricted to Registered users only Download (290kB) | Request a copy
	PDF 12104_2011_9354_MOESM1_ESM.pdf - Published Supplemental Material Restricted to Registered users only Download (104kB) | Request a copy

Abstract

Sequence specific resonance assignments have been obtained for H-1, C-13 and N-15 nuclei of the 21 kDa (188 residues long) glutamine amido transferase subunit of guanosine monophosphate synthetase from Methanocaldococcus jannaschii. From an analysis of H-1 and C-13(alpha), C-13(beta) secondary chemical shifts, (3) JH(N)H(alpha) scalar coupling constants and sequential, short and medium range H-1-H-1 NOEs, it was deduced that the glutamine amido transferase subunit has eleven strands and five helices as the major secondary structural elements in its tertiary structure.

Item Type:	Journal Article
Publication:	BIOMOLECULAR NMR ASSIGNMENTS
Publisher:	SPRINGER
Additional Information:	Copyright for this article belongs to Springer
Keywords:	Amidotransferase; Ammonia channeling; De-novo purine nucleotide biosynthesis; NMR resonance assignments; Secondary structure; Methanocaldococcus jannaschii
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	07 Dec 2012 09:19
Last Modified:	11 Dec 2012 05:58
URI:	http://eprints.iisc.ac.in/id/eprint/45226

Actions (login required)

View Item