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Roles of residues in the interface of transient protein-protein complexes before complexation

Swapna, Lakshmipuram S and Bhaskara, Ramachandra M and Sharma, Jyoti and Srinivasan, Narayanaswamy (2012) Roles of residues in the interface of transient protein-protein complexes before complexation. In: Scientific Reports, 2 .

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Official URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC331220...


Transient protein-protein interactions play crucial roles in all facets of cellular physiology. Here, using an analysis on known 3-D structures of transient protein-protein complexes, their corresponding uncomplexed forms and energy calculations we seek to understand the roles of protein-protein interfacial residues in the unbound forms. We show that there are conformationally near invariant and evolutionarily conserved interfacial residues which are rigid and they account for similar to 65% of the core interface. Interestingly, some of these residues contribute significantly to the stabilization of the interface structure in the uncomplexed form. Such residues have strong energetic basis to perform dual roles of stabilizing the structure of the uncomplexed form as well as the complex once formed while they maintain their rigid nature throughout. This feature is evolutionarily well conserved at both the structural and sequence levels. We believe this analysis has general bearing in the prediction of interfaces and understanding molecular recognition.

Item Type: Journal Article
Publication: Scientific Reports
Publisher: Nature Publishing Group
Additional Information: Copyright of this article belongs to Nature Publishing Group.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 17 Apr 2012 12:21
Last Modified: 17 Apr 2012 12:21
URI: http://eprints.iisc.ac.in/id/eprint/44299

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