A new pentameric structure of rotavirus NSP4 revealed by molecular replacement

Chacko, Anita R and Jeyakanthan, J and Ueno, G and Sekar, K and Rao, Durga C and Dodson, Eleanor J and Suguna, Kaza and Read, Randy J (2012) A new pentameric structure of rotavirus NSP4 revealed by molecular replacement. In: Acta Crystallographica Section D, 68 (Part 1). pp. 57-61.

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Abstract

The region spanning residues 95-146 of the rotavirus nonstructural protein NSP4 from the asymptomatic human strain ST3 has been purified and crystallized and diffraction data have been collected to a resolution of 2.6 angstrom. Several attempts to solve the structure by the molecular-replacement method using the available tetrameric structures of this domain were unsuccessful despite a sequence identity of 73% to the already known structures. A more systematic approach with a dimer as the search model led to an unexpected pentameric structure using the program Phaser. The various steps involved in arriving at this molecular-replacement solution, which unravelled a case of subtle variation between different oligomeric states unknown at the time of solving the structure, are presented in this paper.

Item Type:	Journal Article
Publication:	Acta Crystallographica Section D
Publisher:	International Union of Crystallography
Additional Information:	Copyright of this article belongs to International Union of Crystallography.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	27 Jan 2012 06:24
Last Modified:	27 Jan 2012 06:24
URI:	http://eprints.iisc.ac.in/id/eprint/43136

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