DNA Free Energy-Based Promoter Prediction and Comparative Analysis of Arabidopsis and Rice Genomes

Morey, Czuee and Mookherjee, Sushmita and Rajasekaran, Ganesan and Bansal, Manju (2011) DNA Free Energy-Based Promoter Prediction and Comparative Analysis of Arabidopsis and Rice Genomes. In: Plant Physiology, 156 (3). pp. 1300-1315.

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Abstract

The cis-regulatory regions on DNA serve as binding sites for proteins such as transcription factors and RNA polymerase. The combinatorial interaction of these proteins plays a crucial role in transcription initiation, which is an important point of control in the regulation of gene expression. We present here an analysis of the performance of an in silico method for predicting cis-regulatory regions in the plant genomes of Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa) on the basis of free energy of DNA melting. For protein-coding genes, we achieve recall and precision of 96% and 42% for Arabidopsis and 97% and 31% for rice, respectively. For noncoding RNA genes, the program gives recall and precision of 94% and 75% for Arabidopsis and 95% and 90% for rice, respectively. Moreover, 96% of the false-positive predictions were located in noncoding regions of primary transcripts, out of which 20% were found in the first intron alone, indicating possible regulatory roles. The predictions for orthologous genes from the two genomes showed a good correlation with respect to prediction scores and promoter organization. Comparison of our results with an existing program for promoter prediction in plant genomes indicates that our method shows improved prediction capability.

Item Type:	Journal Article
Publication:	Plant Physiology
Publisher:	American Society of Plant Biologists
Additional Information:	Copyright of this article belongs to American Society of Plant Biologists.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	18 Jul 2011 08:37
Last Modified:	18 Jul 2011 08:37
URI:	http://eprints.iisc.ac.in/id/eprint/39192

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