Protection against UVB inactivation (in vitro) of rat lens enzymes by natural antioxidants

Reddy, Bhanuprakash G and Bhat, Seethraram K (1999) Protection against UVB inactivation (in vitro) of rat lens enzymes by natural antioxidants. In: Molecular and Cellular Biochemistry, 194 (1-2). 41-45 .

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Abstract

Oxidative damage, through increased production of free radicals, is believed to be involved in UV-induced cataractogenesis (eye lens opacification). The possibility of UVB radiation causing damage to important lenticular enzymes was assessed by irradiating 3 months old rat lenses (in RPMI-1640 medium) at 300 nm (100 mu Wcm(-2)) for 24 h, in the absence and presence of ascorbic acid, alpha-tocopherol acetate and beta-carotene. UVB irradiation resulted in decreased activities of hexokinase, glucose-6-phosphate dehydrogenase, aldose reductase, and Na, K- ATPase by 42, 40, 44 and 57% respectively. While endopeptidase activity (229%) and lipid peroxidation (156%) were increased, isocitrate dehydrogenase activity was not altered on irradiation. In the presence of externally added ascorbic acid, tocopherol and beta-carotene (separately) to the medium, the changes in enzyme activities (except endopeptidase) and increased lipid peroxidation, due to UVB exposure, were prevented. These results suggest that UVB radiation exerts oxidative damage on lens enzymes and antioxidants were protective against this damage.

Item Type:	Journal Article
Publication:	Molecular and Cellular Biochemistry
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	UVB-irradiation;lens;enzymes;photoinactivation;ascorbic acid;a-tocopherol;b-carotene
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	28 Jun 2011 10:21
Last Modified:	28 Jun 2011 10:21
URI:	http://eprints.iisc.ac.in/id/eprint/38739

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