Crystallization and preliminary X-ray diffraction analysis of a galactose-specific lectin from the seeds of Butea monosperma

Abhilash, Joseph and Geethanandan, K and Bharath, SR and Sadasivan, C and Haridas, M (2011) Crystallization and preliminary X-ray diffraction analysis of a galactose-specific lectin from the seeds of Butea monosperma. In: Acta Crystallographica Section F, 67 (Part 4). pp. 524-526.

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Abstract

The galactose-specific lectin from the seeds of Butea monosperma has been crystallized by the hanging-drop vapour-diffusion technique. The crystals belonged to space group P1, with unit-cell parameters a = 78.45, b = 78.91, c = 101.85 A, alpha = 74.30, beta = 76.65, gamma = 86.88 degrees. X-ray diffraction data were collected to a resolution of 2.44 A under cryoconditions (100 K) using a MAR image-plate detector system mounted on a rotating-anode X-ray generator. Molecular-replacement calculations carried out using the coordinates of several structures of legume lectins as search models indicate that the galactose-specific lectin from B. monosperma forms an octamer.

Item Type:	Journal Article
Publication:	Acta Crystallographica Section F
Publisher:	John Wiley & Sons
Additional Information:	Copyright of this article belongs to John Wiley & Sons.
Keywords:	galactose;specific lectin;Butea monosperma
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	20 May 2011 07:14
Last Modified:	20 May 2011 07:14
URI:	http://eprints.iisc.ac.in/id/eprint/37694

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