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X-Ray diffraction studies on Mycobacterium smegmatis DNA

Rajagopalan, Malini and Brahmachari, Samir K and Sasisekharan, V and Srivastava, Rakesh (1983) X-Ray diffraction studies on Mycobacterium smegmatis DNA. In: Biopolymers, 22 (7). pp. 1633-1635.


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In this report, we present fiber-diffraction studies on Mycobacterium smegmatis DNA with high GC content (70%). This was done as part of the investigation of mycobacteria as the group which includes some of the dreadful pathogens that cause tuberculosis, leprosy, and many other diseases. Recently,it has been shown that the Mycobacterium smegmatis genomic DNA has two distinct stretches, with different GC contents. Such an arrangement of sequences may be of some physical significance for the structure of DNA and, thereby, of physiological significance. Hence, Mycobacterium smegmatis DNA was subjected to x-ray diffraction studies. We observed that at a 1:l Na+:phosphate concentration, this DNA exhibits a characteristic A-pattern (unlike Mycobacterium tuberculosis3) throughout the humidity range 30-98%.

Item Type: Journal Article
Publication: Biopolymers
Publisher: John Wiley & Sons, Inc.
Additional Information: Copyright of this article belongs to John Wiley & Sons, Inc.
Keywords: Mycobacterium smegmatis;dna;x ray
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 07 Jun 2004
Last Modified: 19 Sep 2010 04:12
URI: http://eprints.iisc.ac.in/id/eprint/369

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