Conformation of polymerizable diacetylenic lecithin, DC8,9PC, by 1H-NMR spectroscopy

Easwaran, KRK and Balasubramanian, SV and Singh, A (1994) Conformation of polymerizable diacetylenic lecithin, DC8,9PC, by 1H-NMR spectroscopy. In: Chemistry and physics of lipids, 69 (2). pp. 161-165.

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Abstract

Diacetylenic phospholipid, 1,2 bis-(tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine (DC8,9PC), forms helices and tubules in addition to liposomes. The diacetylenic moiety responsible for the transformation is probed by 2-D NMR correlated spectroscopy. Chemical shift assignments and the analysis of 2D-COSY measurements were done on the lipid in chloroform-d solution. Based on this analysis, a model for the lipid is proposed. The geometry of the headgroup, glycerol backbone and acyl chains up to three methylenes from glycerol backbone [-(CH2)(3)-] is similar to that of dipalmitoyl phosphatidylcholine. The estimated torsional angle for methylene groups adjacent to diacetylenic moieties suggested an overall tilt of the diacetylenic lipid molecule from the bilayer axis of 25-30 degrees. This tilt could be negative or positive depending on the handedness of the resultant microstructures.

Item Type:	Editorials/Short Communications
Publication:	Chemistry and physics of lipids
Publisher:	Elsevier science
Additional Information:	Copyright of this article belongs to Elsevier science.
Keywords:	Polymerizable phospholipid; Nuclear magnetic resonance; Correlated spectroscopy; Lipid conformation;Tubules
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	26 Mar 2011 04:45
Last Modified:	26 Mar 2011 04:45
URI:	http://eprints.iisc.ac.in/id/eprint/36302

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