A23187—Channel behaviour: Fluorescence study

Jyothi, G and Surolia , A and Easwaran, KRK (1994) A23187—Channel behaviour: Fluorescence study. In: Journal of Biosciences, 19 (3). pp. 277-282. (Submitted)

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Abstract

Pyranine entrapped soylipid liposomes have been used as a model system to study the proton transport across membrane in the presence of A23187, a carboxylic ionophore specific for electroneutral exchange of divalent cations. An apparent rate constant (k(app)) for transport of protons has been determined from the rate of change of fluorescence intensity of pyranine by stopped flow rapid kinetics in the presence of proton gradient. The variation of k(app) has been studied as a function of ionophore concentration and the results have been compared with gramicidin-a well known channel former under the similar experimental conditions. The rates thus obtained showed that A23187 is not only a simple carrier but also shows channel behaviour at high concentration of ionophore.

Item Type:	Journal Article
Publication:	Journal of Biosciences
Publisher:	Indian Academy of Sciences
Additional Information:	Copyright of this article belongs to Indian Academy of Sciences.
Keywords:	A23187—calcium ionophore;proton transport;stopped flow k
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	03 Jun 2011 08:04
Last Modified:	03 Jun 2011 08:04
URI:	http://eprints.iisc.ac.in/id/eprint/36090

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