Crystallization and preliminary X-ray studies of a galactose-specific lectin from the seeds of bitter gourd (Momordica charantia)

Chandran, Thyageshwar and Sharma, Alok and Vijayan, M (2010) Crystallization and preliminary X-ray studies of a galactose-specific lectin from the seeds of bitter gourd (Momordica charantia). In: Acta Crystallographica Section F, 66 (Part 9). pp. 1037-1040.

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Abstract

A galactose-specific lectin from the seeds of bitter gourd (Momordica charantia) is a four-chain type II ribosome-inactivating protein (RIP) resulting from covalent association through a disulfide bridge between two identical copies of a two-chain unit. The available structural information on such four-chain RIPs is meagre. The bitter gourd lectin was therefore crystallized for structural investigation and the crystals have been characterized. It is anticipated that the structure of the orthorhombic crystals will be analysed using molecular replacement by taking advantage of its sequence, and presumably structural, homology to normal two-chain type II RIPs.

Item Type:	Journal Article
Publication:	Acta Crystallographica Section F
Publisher:	John Wiley and Sons
Additional Information:	Copyright of this article belongs to John Wiley and Sons.
Keywords:	Galactose-specific lectins; bitter gourd; Momordica charantia; ribosome-inactivating proteins.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	27 Sep 2010 11:35
Last Modified:	27 Sep 2010 11:35
URI:	http://eprints.iisc.ac.in/id/eprint/32491

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