Rao, Narasinga BN and Ramakrishnan, C and Balaram, P (1979) Metal binding to pyridoxal derivatives. An NMR study of the interaction of Eu(III) with pyridoxal phosphate and pyridoxamine phosphate. In: Journal of Biosciences, 1 (1). pp. 35-47.
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Abstract
The solution conformations of pyridoxal-5’-phosphate and pyridoxamine-5’-phosphate have been investigated using Eu(III) as a nuclear magnetic resonance shift probe. Binding of Eu(III) to pyridoxal phosphate results in the formation of two complexes, at the phosphate group and the \alpha-hydroxy-aldehyde moiety, which are in slow exchange on the nuclear magnetic resonance time-scale. The lanthanide-induced pseudo contact shifts calculated using the McConnell-Robertson equation. (J. Chem. Soc. (1950), 22, 1561) are in good agreement with the experimentally observed values for both pyridoxal phosphate and pyridoxamine phosphate and lead to a family of closely related conformations.
Item Type: | Journal Article |
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Publication: | Journal of Biosciences |
Publisher: | Indian Academy of Sciences |
Additional Information: | Copyright for this article belongs to Indian Academy of Sciences. |
Keywords: | conformation;lanthanide;nuclear magnetic resonance;pyridoxal-5’-phosphate;pyridoxamine-5’-phosphate |
Department/Centre: | Division of Biological Sciences > Molecular Biophysics Unit |
Date Deposited: | 12 May 2005 |
Last Modified: | 19 Sep 2010 04:18 |
URI: | http://eprints.iisc.ac.in/id/eprint/3182 |
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