Crystallization and preliminary X-ray crystallographic analysis of a galactose-specific lectin from Dolichos lablab

Latha, VL and Kulkarni, KA and Rao, RN and Kumar, NS and Suguna, K (2006) Crystallization and preliminary X-ray crystallographic analysis of a galactose-specific lectin from Dolichos lablab. In: Acta Crystallographica Section F-Structural Biology And Crystallization Communications, 62 (Part 2). pp. 163-165.

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Abstract

The galactose-specific lectin from the seeds of Dolichos lablab has been crystallized using the hanging-drop vapour-diffusion technique. The crystals belong to space group P1, with unit-cell parameters a = 73.99, b = 84.13, c = 93.15 angstrom, alpha = 89.92, beta = 76.01, gamma = 76.99 degrees. X-ray diffraction data to a resolution of 3.0 angstrom have been collected under cryoconditions ( 100 K) using a MAR imaging-plate detector system mounted on a rotating-anode X-ray generator. Molecular-replacement calculations carried out using the available structures of legume lectins as search models revealed that the galactose-specific lectin from D. lablab forms a tetramer similar to soybean agglutinin; two such tetramers are present in the asymmetric unit.

Item Type:	Journal Article
Publication:	Acta Crystallographica Section F-Structural Biology And Crystallization Communications
Publisher:	International Union of Crystallography
Additional Information:	Copyright of this article belongs to International Union of Crystallography.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	16 Sep 2010 06:53
Last Modified:	19 Sep 2010 06:15
URI:	http://eprints.iisc.ac.in/id/eprint/31783

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