Synthesis of Homologous Fluorescent Carboxylates and their Application to the Study of Bovine Serum Albumin

Narayanan, Revathi and Balaram, P (1980) Synthesis of Homologous Fluorescent Carboxylates and their Application to the Study of Bovine Serum Albumin. In: Bioorganic Chemistry, 9 (3). pp. 352-362.

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Abstract

The synthesis of four fluorescent carboxylate probes with spacer groups comprising 1 to 12 atoms between the dansyl and carboxylate functions is described. These probes bind specifically to bovine serum albumin with changes in fluorescence properties. Dansyl glycine shows significantly different behavior in comparison with its longer homologs. Probe binding to protein is diminished in the presence of fatty acids. Evaluation of binding stoichiometries, dissociation constants, and limiting fluorescence values suggests that dansyl glycine binds at a site distinct from the binding site for the longer homologs.

Item Type:	Journal Article
Publication:	Bioorganic Chemistry
Publisher:	Elsevier
Additional Information:	Copyright for this article belongs to Elsevier Science Ltd.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	08 Apr 2005
Last Modified:	19 Sep 2010 04:18
URI:	http://eprints.iisc.ac.in/id/eprint/3054

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