Comparative analysis of thermophilic and mesophilic proteins using Protein Energy Networks

Vijayabaskar, MS and Vishveshwara, Saraswathi (2010) Comparative analysis of thermophilic and mesophilic proteins using Protein Energy Networks. In: BMC Bioinformatics, 11 (Suppl.).

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Abstract

Background: Thermophilic proteins sustain themselves and function at higher temperatures. Despite their structural and functional similarities with their mesophilic homologues, they show enhanced stability. Various comparative studies at genomic, protein sequence and structure levels, and experimental works highlight the different factors and dominant interacting forces contributing to this increased stability. Methods: In this comparative structure based study, we have used interaction energies between amino acids, to generate structure networks called as Protein Energy Networks (PENs). These PENs are used to compute network, sub-graph, and node specific parameters. These parameters are then compared between the thermophile-mesophile homologues. Results: The results show an increased number of clusters and low energy cliques in thermophiles as the main contributing factors for their enhanced stability. Further more, we see an increase in the number of hubs in thermophiles. We also observe no community of electrostatic cliques forming in PENs. Conclusion: In this study we were able to take an energy based network approach, to identify the factors responsible for enhanced stability of thermophiles, by comparative analysis. We were able to point out that the sub-graph parameters are the prominent contributing factors. The thermophiles have a better-packed hydrophobic core. We have also discussed how thermophiles, although increasing stability through higher connectivity retains conformational flexibility, from a cliques and communities perspective.

Item Type:	Journal Article
Publication:	BMC Bioinformatics
Publisher:	BioMed Central
Additional Information:	Copyright of this article belongs to BioMed Central.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	26 May 2010 07:27
Last Modified:	19 Sep 2010 06:07
URI:	http://eprints.iisc.ac.in/id/eprint/28037

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