Patanjali, Sankhavaram R and Sajjan, Sajjan U and Surolia, Avadhesha (1988) Erythrocyte-binding studies on an acidic lectin from winged bean (Psophocarpus tetragonolobus). In: Biochemical Journal, 252 (3). pp. 625-631.
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n acidic lectin (WBA II) was isolated to homogeneity from the crude seed extract of the winged bean (Psophocarpus tetragonolobus) by affinity chromatography on lactosylaminoethyl-Bio-Gel. Binding of WBA II to human erythrocytes of type-A, -B and -O blood groups showed the presence of 10(5) receptors/cell, with high association constants (10(6)-10(8) M-1). Competitive binding studies with blood-group-specific lectins reveal that WBA II binds to H- and T-antigenic determinants on human erythrocytes. Affinity-chromatographic studies using A-, B-, H- and T-antigenic determinants coupled to an insoluble matrix confirm the specificity of WBA II towards H- and T-antigenic determinants. Inhibition of the binding of WBA II by various sugars show that N-acetylgalactosamine and T-antigenic disaccharide (Thomsen-Friedenreich antigen, Gal beta 1-3GalNAc) are the most potent mono- and di-saccharide inhibitors respectively. In addition, inhibition of the binding of WBA II to erythrocytes by dog intestine H-fucolipid prove that the lectin binds to H-antigenic determinant.
| Item Type: | Journal Article |
|---|---|
| Publication: | Biochemical Journal |
| Publisher: | Portland Press |
| Additional Information: | Copyright of this article belongs to Portland Press. |
| Department/Centre: | Division of Biological Sciences > Molecular Biophysics Unit |
| Date Deposited: | 18 May 2010 10:13 |
| Last Modified: | 18 May 2010 10:13 |
| URI: | http://eprints.iisc.ac.in/id/eprint/27540 |
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