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Synthetic arabinomannan glycolipids and their effects on growth and motility of the Mycobacterium smegmatis

Naresh, Kottari and Bharati, Binod Kumar and Avaji, Prakash Gouda and Jayaraman, Narayanaswamy and Chatterji, Dipankar (2010) Synthetic arabinomannan glycolipids and their effects on growth and motility of the Mycobacterium smegmatis. In: Organic & Biomolecular Chemistry, 8 (3). pp. 592-599.

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Arabinomannan-containing glycolipids, relevant to the mycobacterial cell-wall component lipoarabinomannan, were synthesized by chemical methods. The glycolipids were presented with tri- and tetrasaccharide arabinomannans as the sugar portion and a double alkyl chain as the lyophilic portion. Following synthesis, systematic biological and biophysical studies were undertaken in order to identify the effects of the glycolipids during mycobacterium growth. The studies included mycobacterial growth, biofilm formation and motility assays. From the studies, it was observed that the synthetic glycolipid with higher arabinan residues inhibited the mycobacterial growth, lessened the biofilm formation and impaired the motility of mycobacteria. A surface plasmon resonance study involving the immobilized glycan surface and the mycobacterial crude lysates as analytes showed specificities of the interactions. Further, it was found that cell lysates from motile bacteria bound oligosaccharide with higher affinity than non-motile bacteria.

Item Type: Journal Article
Publication: Organic & Biomolecular Chemistry
Publisher: Royal Society of chemistry
Additional Information: Copyright for this article belongs to Royal Society of chemistry.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Division of Chemical Sciences > Organic Chemistry
Date Deposited: 09 Feb 2010 11:27
Last Modified: 19 Sep 2010 05:54
URI: http://eprints.iisc.ac.in/id/eprint/25504

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