A single point mutation disrupts the capsid assembly in Sesbania Mosaic Virus resulting in a stable isolated dimer

Pappachan, Anju and Chinnathambi, Subashchandrabose and Satheshkumar, PS and Savithri, HS and Murthy, MRN (2009) A single point mutation disrupts the capsid assembly in Sesbania Mosaic Virus resulting in a stable isolated dimer. In: Virology, 392 (2). pp. 215-221.

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Abstract

Protein-protein interactions play a Crucial role in Virus assembly and stability. With the view of disrupting capsid assembly and capturing smaller oligomers, interfacial residue mutations were carried Out in the coat protein gene of Sesbania Mosaic Virus, a T=3 ss (+) RNA plant virus. A single point mutation of a Trp 170 present at the five-fold interface of the virus to a charged residue (Glu or Lys) arrested assembly of virus like particles and resulted in stable Soluble dimers of the capsid Protein. The X-ray crystal structure of one of the isolated dimer mutants - rCP Delta N65W170K was determined to a resolution of 2.65 angstrom. Detailed analysis of the dimeric mutant protein structure revealed that a number of Structural changes take place, especially in the loop and interfacial regions during the course of assembly. The isolated chiller was ``more relaxed'' than the dimer found in the T=3 or T=1 capsids. The isolated dimer does not bind Ca2+ ion and consequently four C-terminal residues are disordered. The FG loop, which interacts with RNA in the Virus, has different conformations in the isolated dimer and the intact Virus Suggesting its flexible nature and the conformational changes that accompany assembly. The isolated choler mutant was much less stable when compared to the assembled capsids, suggesting the importance of inter-subunit interactions and Ca2+ mediated interactions in the stability of the capsids. With this study, SeMV becomes the first icosahedral virus for which X-ray crystal Structures of T=3, T=1 capsids as well as a smaller oligomer of the capsid protein have been determined.

Item Type:	Journal Article
Publication:	Virology
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Inc.
Keywords:	Virus capsid; Virus assembly; Virus stability; Ca2+; Protein-protein interactions; X-ray crystallography
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	06 Jan 2010 10:15
Last Modified:	19 Sep 2010 05:49
URI:	http://eprints.iisc.ac.in/id/eprint/24203

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