Vijayasarathy, S and Balaram, P (1982) Regional differentiation in bull sperm plasma-membranes. In: Biochemical and Biophysical Research Communications, 108 (2). pp. 760-769.
![]() |
PDF
science.pdf - Published Version Restricted to Registered users only Download (1MB) | Request a copy |
Abstract
Bull sperm heads and tails have been separated by proteolytic digestion (trypsin) and plasma membranes have been isolated, using discontinuous sucrose density gradient centrifugation. Plasma membrane bound Ca2+-ATPase is shown to be associated mostly with the tail membranes. Pyrene excimer fluorescence and diphenylhexatriene fluorescence polarization experiments indicate a more fluid lipid phase in the tail region. Differences in surface charge distribution have been found, using 1-anilinonaphthalene-8-sulfonate and Tb3+ as fluorescent probes.
Item Type: | Journal Article |
---|---|
Publication: | Biochemical and Biophysical Research Communications |
Publisher: | Elsevier Science |
Additional Information: | Copyright of this article belongs to Elsevier Science. |
Department/Centre: | Division of Biological Sciences > Molecular Biophysics Unit |
Date Deposited: | 30 Dec 2009 11:28 |
Last Modified: | 19 Sep 2010 05:41 |
URI: | http://eprints.iisc.ac.in/id/eprint/22404 |
Actions (login required)
![]() |
View Item |