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Homologous recombination in mycobacteria

Muniyappa, K and Ganesh, N and Guhan, N and Singh, Pawan and Manjunath, GP and Datta, S and Chandra, Nagasuma R and Vijayan, M (2004) Homologous recombination in mycobacteria. In: Current Science, 86 (1). pp. 141-148.


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In recent years, considerable effort and resources have been expended to develop targeted gene delivery methods, and generation of auxotrophic mutants of mycobacteria. The results of these studies suggest that mycobacteria exhibit a wide range of recombination rates, which vary from loci to loci. Here we review the methods developed for allele exchange and targeted gene disruption as well as the mechanistic aspects of homologous recombination in mycobacteria. The results of whole genome, functional and structural analyses of Mycobacterium tuberculosis and Mycobacterium smegmatis RecA and SSB proteins provide insights into variations of the prototypic Escherichia coli paradigm. This variation of a common theme might allow mycobacteria to function in their natural but complex physiological environments.

Item Type: Journal Article
Publication: Current Science
Publisher: Indian Academy of Sciences
Additional Information: The copyright belongs to Indian Academy of Sciences
Keywords: homologous;recombination;mycobacteria;auxotrophic mutants
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Division of Information Sciences (Doesn't exist now) > BioInformatics Centre
Division of Biological Sciences > Biochemistry
Date Deposited: 28 Oct 2004
Last Modified: 19 Sep 2010 04:17
URI: http://eprints.iisc.ac.in/id/eprint/2204

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