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‘‘Teflon-Coated Peptides’’: Hexafluoroacetone Trihydrate as a Structure Stabilizer for Peptides

Rajan, Rahul and Awasthi, Satish Kumar and Bhattacharjya, Surajit and Balaram, P (1997) ‘‘Teflon-Coated Peptides’’: Hexafluoroacetone Trihydrate as a Structure Stabilizer for Peptides. In: Biopolymers, 42 (2). pp. 125-128.


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In the last 30 years, since the early work of Goodman and co-workers introducing trifluoroethanol as a solvent in peptide conformational analysis,1,2 the ability of fluoroalcohols to stabilize secondary structures in peptides in aqueous solution is well documented.3–7 However, the detailed molecular mechanisms remain to be understood. 3,4,8–12 2,2,2-Trifluoroethanol (TFE) has been the most widely used fluoroalcohol additive to aqueous solutions of peptides and proteins.4,13–18 We describe in this communication the superior structure-stabilization properties of hexafluoroacetone trihydrate (HFA, a covalent hydrate, of the ketone, a gem diol, hexafluoropropan-2,2- diol) 19–21 and provide a rationale for the induction of intramolecularly hydrogen-bonded conformations in peptides, in aqueous fluoroalcohol solutions.

Item Type: Journal Article
Publication: Biopolymers
Publisher: John Wiley & Sons, Inc
Additional Information: Copyright for this article belongs to John Wiley & Sons, Inc.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 28 Oct 2004
Last Modified: 26 Feb 2019 08:49
URI: http://eprints.iisc.ac.in/id/eprint/2200

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