Bull sperm plasma and acrosomal membranes: Fluorescence studies of lipid phase fluidity

Vijayasarathy, S and Shivaji, S and Balaram, P (1982) Bull sperm plasma and acrosomal membranes: Fluorescence studies of lipid phase fluidity. In: Biochemical and Biophysical Research Communications, 108 (2). pp. 585-591.

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Abstract

Bull sperm plasma and outer acrosomal membranes have been isolated by discontinuous sucrose density gradient centrifugation and Ca2+-ATPase activity has been determined for both the membranes. Pyrene excimer fluorescence and diphenylhexatriene fluorescence polarization studies show that the lipid phase of the sperm plasma membranes is more fluid than the lipids of the outer acrosomal membranes. Approximately, a three fold increase in the cholesterol content has been found in the outer acrosomal membranes as compared to that in the plasma membranes.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	01 Jul 2009 10:43
Last Modified:	19 Sep 2010 05:35
URI:	http://eprints.iisc.ac.in/id/eprint/20903

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