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Tissue culture of forest trees: Clonal multiplication of Eucalyptus grandis L

Rao, K.Sankara and Venkateswara, R (1985) Tissue culture of forest trees: Clonal multiplication of Eucalyptus grandis L. In: Plant Science, 40 (1). pp. 51-55.

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Axillary shoot proliferation was obtained using explants of Eucalyptus grandis L. juvenile and mature stages on a defined medium. Murashige and Skoog medium (MS) supplemented with benzyladenine (BA), naphthalene acetic acid (NAA) and additional thiamine. Excised shoots were induced to root on a sequence of three media: (1) White's medium containing indoleacetic acid (IAA), NAA and indole butyric acid; (IBA), (2) half-strength MS medium with charcoal and (3) half-strength MS liquid medium. The two types of explants differed in rooting response, with juvenile-derived shoots giving 60% rooting and adult-derived ones only 35%. Thus, the factors limiting cloning of selected trees in vitro are determined to be those controlling rooting of shoots in E. grandis.

Item Type: Journal Article
Publication: Plant Science
Publisher: Elsevier Science
Additional Information: The copyright of this article belongs to Elsevier Science
Keywords: Eucalyptus grandis L.; clonal multiplication; axillary shoot proliferation; tissue cultures.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 17 Nov 2009 13:04
Last Modified: 19 Sep 2010 05:33
URI: http://eprints.iisc.ac.in/id/eprint/20487

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