Tissue culture of forest trees: Clonal multiplication of Eucalyptus grandis L

Rao, K.Sankara and Venkateswara, R (1985) Tissue culture of forest trees: Clonal multiplication of Eucalyptus grandis L. In: Plant Science, 40 (1). pp. 51-55.

PDF pdf.pdf - Published Version Restricted to Registered users only Download (385kB) | Request a copy

Abstract

Axillary shoot proliferation was obtained using explants of Eucalyptus grandis L. juvenile and mature stages on a defined medium. Murashige and Skoog medium (MS) supplemented with benzyladenine (BA), naphthalene acetic acid (NAA) and additional thiamine. Excised shoots were induced to root on a sequence of three media: (1) White's medium containing indoleacetic acid (IAA), NAA and indole butyric acid; (IBA), (2) half-strength MS medium with charcoal and (3) half-strength MS liquid medium. The two types of explants differed in rooting response, with juvenile-derived shoots giving 60% rooting and adult-derived ones only 35%. Thus, the factors limiting cloning of selected trees in vitro are determined to be those controlling rooting of shoots in E. grandis.

Item Type:	Journal Article
Publication:	Plant Science
Publisher:	Elsevier Science
Additional Information:	The copyright of this article belongs to Elsevier Science
Keywords:	Eucalyptus grandis L.; clonal multiplication; axillary shoot proliferation; tissue cultures.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	17 Nov 2009 13:04
Last Modified:	19 Sep 2010 05:33
URI:	http://eprints.iisc.ac.in/id/eprint/20487

Actions (login required)

View Item