Ghatpande, AS and Sikdar, SK (1997) Competition for binding between veratridine and KIFMK: An open channel blocking peptide of the RIIA sodium channel. In: Journal of Membrane Biology, 160 (3). pp. 177-182.
PDF
Competition.pdf - Published Version Restricted to Registered users only Download (664kB) | Request a copy |
Abstract
Veratridine, an alkaloid isolated from the rhizome of V. album, binds and slows the inactivation of the brain sodium channels. The synthetic pentapeptide KIFMK causes a voltage-and use-dependent open-channel block of the RIIA (rat brain type IIA) sodium channel (Eaholtz, Scheuer & Catterall, 1994). Our stud ies on the RIIA sodium channel expressed in CHO cells reveal that the fraction of veratridine modified sodium channels decreases linearly with increasing KIFMK concentration. However, the time constant for dissociation of veratridine from the channel remains unchanged in the presence of a high concentration of KIFMK, as opposed to that in the presence of QX314 where the dissociation appears to be more complex. These data are consistent with mutually exclusive binding of the open channel blocking peptide and veratridine to the brain sodium channel.
Item Type: | Journal Article |
---|---|
Publication: | Journal of Membrane Biology |
Publisher: | Springer Verlag |
Additional Information: | Copyright of this article belongs to Springer Verlag. |
Keywords: | Veratridine;KIFMK;sodium channel;binding competition;site 2receptor;whole cell patch clamp. |
Department/Centre: | Division of Biological Sciences > Molecular Biophysics Unit |
Date Deposited: | 11 Mar 2009 09:53 |
Last Modified: | 19 Sep 2010 05:25 |
URI: | http://eprints.iisc.ac.in/id/eprint/18714 |
Actions (login required)
View Item |