Detection of putative Zn(II) binding sites within Escherichia coli RNA polymerase: inconsistency between sequence-based prediction and $^{65}Zn$ blotting

Sujatha, Sitaraman and Chatterji, Dipankar (1999) Detection of putative Zn(II) binding sites within Escherichia coli RNA polymerase: inconsistency between sequence-based prediction and $^{65}Zn$ blotting. In: Federation of European Biochemical Societies Letters, 454 (1-2). pp. 169-171.

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Abstract

The availability of repeating 'Cys' and/or 'His' units in a particular order prompts the prediction of Zn(II) finger motifs in a protein. Escherichia coli RNA polymerase has two tightly bound Zn(II) per molecule of the enzyme as detected by atomic absorption spectroscopy. One Zn(II) was identified to be at the beta subunit, whereas the other putative Zn(II) binding site has recently been predicted to be at the N-terminal half of the beta' subunit, from primary sequence analysis. We show here that the beta' subunit has no ability to bind Zn-65(II). On the other hand, the N-terminal domain of the a subunit has strong Zn(II) binding ability with no obvious functional implications. (C) 1999 Federation of European Biochemical Societies.

Item Type:	Journal Article
Publication:	Federation of European Biochemical Societies Letters
Publisher:	Federation of European Biochemical Societies
Additional Information:	Copyright of this article belongs to Federation of European Biochemical Societies.
Keywords:	Zn(II) site;Zn(II) finger;65Z blotting;Atomic absorption; Subunit location.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	06 Mar 2009 08:59
Last Modified:	19 Sep 2010 05:01
URI:	http://eprints.iisc.ac.in/id/eprint/18387

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