Defining substrate interactions with calreticulin: an isothermal titration calorimetric study

Gupta, Garima and Gemma, Emiliano and Oscarson, Stefan and Surolia, Avadhesha (2008) Defining substrate interactions with calreticulin: an isothermal titration calorimetric study. In: Glycoconjugate Journal, 25 (8). pp. 797-802.

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Abstract

Calreticulin (CRT) is a soluble, lectin chaperone found in the endoplasmic reticulum of eukaryotes. It binds the N-glycosylated polypeptides via the glycan intermediate $Glc1Man_{5-9}GlcNAc_{2}$, present on the target glycoproteins. Earlier we have studied interactions of substrate with CRT by isothermal titration calorimetry (ITC) and molecular modeling, to establish that CRT recognizes the Glcα1–3 linkage and forms contacts with each saccharide moiety of the oligosaccharide Glcα1–3Manα1–2Manα1–2Man. We also delineated the amino acid residues in the sugar binding pocket of CRT that play a crucial role in sugar–CRT binding. Here, we have used mono-deoxy analogues of the trisaccharide unit Glcα1–3Manα1–2Man to determine the role of various hydroxyl groups of the sugar substrate in sugar– CRT interactions. Using the thermodynamic data obtained by ITC with these analogues we demonstrate that the 3-OH group of Glc1 plays an important role in sugar–CRT binding, whereas the 6-OH group does not. Also, the 4- OH, 6-OH of Man2 and 3-OH, 4-OH of Man3 in the trisaccharide are involved in binding, of which 6-OH of Man2 and 4-OH of Man3 have a more significant role to play. This study sheds light further on the interactions between the substrate sugar of glycoproteins and the lectin chaperone CRT.

Item Type:	Journal Article
Publication:	Glycoconjugate Journal
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	Calreticulin;Isothermal titration calorimetry;Sugar–CRT interaction.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	18 Dec 2008 10:41
Last Modified:	19 Sep 2010 04:52
URI:	http://eprints.iisc.ac.in/id/eprint/16475

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